Motivation - Why do we care?

·       top level

§       betterment of human civilization, cure diseases

§       learn information about life on this planet

·       The nitty gritty -

§       determine if new DNA sequences were already deposited in databanks

§       find proteins homologous to a putative coding ORF

§       find repeat elements or regulatory sequences

§       locate false priming sites for a set of PCR oligonucleotides

·       evolution

§       all things related, if you learn about one organism, that knowledge can be applied to other organisms

§       which organisms?

·       Model Organisms

§       Useful tool as alternate experimentation on humans

§       because evolution exists experiments can be performed on simpler organisms and the results extracted to apply to humankind


Homology

Homologous sequence is one which is similar to another sequence and the similarity is derived from common ancestry.

Homologous proteins are similar in their folding or structure

Generally homology infers sequence similarity to a high degree, but this is not always the case

Homology searches are a useful tool to narrow your search for future experiments