ID   GRAA_HUMAN     STANDARD;      PRT;   262 AA.
AC   P12544;
DT   01-OCT-1989 (Rel. 12, Created)
DT   01-OCT-1989 (Rel. 12, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   GRANZYME A PRECURSOR (EC 3.4.21.78) (CYTOTOXIC T-LYMPHOCYTE PROTEINASE
DE   1) (HANUKKAH FACTOR) (H FACTOR) (HF) (GRANZYME 1) (CTL TRYPTASE)
DE   (FRAGMENTIN 1).
GN   GZMA OR CTLA3 OR HFSP.
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
RN   [1]
RP   SEQUENCE FROM N.A.
RC   TISSUE=T-cell;
RX   MEDLINE; 88125000.
RA   Gershenfeld H.K., Hershberger R.J., Shows T.B., Weissman I.L.;
RT   "Cloning and chromosomal assignment of a human cDNA encoding a T
RT   cell- and natural killer cell-specific trypsin-like serine
RT   protease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:1184-1188(1988).
RN   [2]
RP   SEQUENCE OF 29-53.
RX   MEDLINE; 88330824.
RA   Poe M., Bennett C.D., Biddison W.E., Blake J.T., Norton G.P.,
RA   Rodkey J.A., Sigal N.H., Turner R.V., Wu J.K., Zweerink H.J.;
RT   "Human cytotoxic lymphocyte tryptase. Its purification from granules
RT   and the characterization of inhibitor and substrate specificity.";
RL   J. Biol. Chem. 263:13215-13222(1988).
RN   [3]
RP   SEQUENCE OF 29-40, AND CHARACTERIZATION.
RX   MEDLINE; 89009866.
RA   Hameed A., Lowrey D.M., Lichtenheld M., Podack E.R.;
RT   "Characterization of three serine esterases isolated from human IL-2
RT   activated killer cells.";
RL   J. Immunol. 141:3142-3147(1988).
RN   [4]
RP   SEQUENCE OF 29-39, AND CHARACTERIZATION.
RX   MEDLINE; 89035468.
RA   Kraehenbuhl O., Rey C., Jenne D.E., Lanzavecchia A., Groscurth P.,
RA   Carrel S., Tschopp J.;
RT   "Characterization of granzymes A and B isolated from granules of
RT   cloned human cytotoxic T lymphocytes.";
RL   J. Immunol. 141:3471-3477(1988).
RN   [5]
RP   3D-STRUCTURE MODELING.
RX   MEDLINE; 89184501.
RA   Murphy M.E.P., Moult J., Bleackley R.C., Gershenfeld H.,
RA   Weissman I.L., James M.N.G.;
RT   "Comparative molecular model building of two serine proteinases from
RT   cytotoxic T lymphocytes.";
RL   Proteins 4:190-204(1988).
CC   -!- FUNCTION: THIS ENZYME IS NECESSARY FOR TARGET CELL LYSIS IN CELL-
CC       MEDIATED IMMUNE RESPONSES. IT CLEAVES AFTER LYS OR ARG. MAY BE
CC       INVOLVED IN APOPTOSIS.
CC   -!- CATALYTIC ACTIVITY: HYDROLYSIS OF PROTEINS, INCLUDING FIBRONECTIN,
CC       TYPE IV COLLAGEN AND NUCLEOLIN. PREFERENTIAL CLEAVAGE: ARG-|-XAA,
CC       LYS-|-XAA >> PHE-|-XAA IN SMALL MOLECULE SUBSTRATES.
CC   -!- SUBUNIT: HOMODIMER, DISULFIDE-LINKED.
CC   -!- SUBCELLULAR LOCATION: CYTOPLASMIC GRANULES.
CC   -!- SIMILARITY: BELONGS TO PEPTIDASE FAMILY S1; ALSO KNOWN AS THE
CC       TRYPSIN FAMILY. STRONGEST TO OTHER GRANZYMES AND TO MAST CELL
CC       PROTEASES.
DR   EMBL; M18737; AAA52647.1; -.
DR   PIR; A28943; A28943.
DR   PIR; A30525; A30525.
DR   PIR; A30526; A30526.
DR   PIR; A31372; A31372.
DR   PDB; 1HF1; 15-OCT-94.
DR   MIM; 140050; -.
DR   INTERPRO; IPR001254; -.
DR   PFAM; PF00089; trypsin; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
KW   Hydrolase; Serine protease; Zymogen; Signal; T-cell; Cytolysis;
KW   Apoptosis; 3D-structure.
FT   SIGNAL        1     26
FT   PROPEP       27     28       ACTIVATION PEPTIDE.
FT   CHAIN        29    262       GRANZYME A.
FT   ACT_SITE     69     69       CHARGE RELAY SYSTEM (BY SIMILARITY).
FT   ACT_SITE    114    114       CHARGE RELAY SYSTEM (BY SIMILARITY).
FT   ACT_SITE    212    212       CHARGE RELAY SYSTEM (BY SIMILARITY).
FT   DISULFID     54     70       BY SIMILARITY.
FT   DISULFID    148    218       BY SIMILARITY.
FT   DISULFID    179    197       BY SIMILARITY.
FT   DISULFID    208    234       BY SIMILARITY.
FT   CARBOHYD    170    170       N-LINKED (GLCNAC...) (POTENTIAL).
SQ   SEQUENCE   262 AA;  28968 MW;  DA87363A0D92BAF4 CRC64;
     MRNSYRFLAS SLSVVVSLLL IPEDVCEKII GGNEVTPHSR PYMVLLSLDR KTICAGALIA
     KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE FPYPCYDPAT REGDLKLLQL
     TEKAKINKYV TILHLPKKGD DVKPGTMCQV AGWGRTHNSA SWSDTLREVN ITIIDRKVCN
     DRNHYNFNPV IGMNMVCAGS LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP
     GVYILLSKKH LNWIIMTIKG AV
//

Each  line begins with a two-character line code, which indicates the type
of  data contained in the line. The current line types and line codes  and
the order in which they appear in an entry, are shown in the table below.


---------  ----------------------------    ----------------------
Line code  Content                         Occurrence in an entry
---------  ----------------------------    ----------------------
ID         Identification                  Once; starts the entry
AC         Accession number(s)             One or more
DT         Date                            Three times
DE         Description                     One or more
GN         Gene name(s)                    Optional
OS         Organism species                One or more
OG         Organelle                       Optional
OC         Organism classification         One or more
RN         Reference number                One or more
RP         Reference position              One or more
RC         Reference comment(s)            Optional
RX         Reference cross-reference(s)    Optional
RA         Reference authors               One or more
RT         Reference title                 Optional
RL         Reference location              One or more
CC         Comments or notes               Optional
DR         Database cross-references       Optional
KW         Keywords                        Optional
FT         Feature table data              Optional
SQ         Sequence header                 Once
           (blanks) sequence data          One or more
//         Termination line                Once; ends the entry
---------  ----------------------------    ----------------------



Feature table keys

.1 Change indicators
.2 Amino acid modifications
.3 Regions
.4 Secondary structure
.5 Others

Most useful annotation about a protein can be found in either comment line or
feature table line, there are also several conserved words used to describe the 
derivation of such information, see more in  entry format.

back to top .